染料名称 Excitation(nm) Emission(nm) 分子量 备注信息 Reactive and conjugated probes 445 410 423 455 551 528 539 386578670 Aminocoumarin 350 Methoxycoumarin 360 Cascade Blue (375);401 Pacific Blue 403 Pacific Orange 403 Lucifer yellow 425 NBD 466 Hydroxycoumarin325R-Phycoerythrin (PE) 330 317 596 406 294 331240 k Succinimidyl ester Succinimidyl ester Hydrazide Maleimide NBD-X Succinimidyl ester 480;565 PE-Cy7 conjugates 480;565;743 Red 613 480;565 PerCP 490 TruRed 490,675 FluorX 494 Fluorescein 495 BODIPY-FL 503 TRITC 547 X-Rhodamine 570 PE-Cy5 conjugates480;565;650Lissamine Rhodamine BTexas Red 767 613 675 695 520 519 512 572 576 590615660 aka Cychrome, R670, Tri-Color, Quantum Red PE-Texas RedPeridinin chlorphyll proteinPerCP-Cy5.5 conjugateGE Healthcare 389 444 548 587 FITC; pH sensitive XRITC TRITC 570589650 625Sulfonyl chloride Allophycocyanin (APC) 104 k APC-Cy7 conjugates650;755 767 PharRedAlexa Fluor系列荧光染料 Alexa Fluor 405 Alexa Fluor 430 Alexa Fluor 488 Alexa Fluor 500 Alexa Fluor 514 Alexa Fluor 532 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 350 401 434 499 503 517 530 561 553 579 343 421 540 519 525 542 555 572 568 603 442 1028 702 643 700 714 724 1079 1250 792 410 QY 0.92 QY 0.79 QY 0.1 QY 0.69 QY 0.61 Alexa Fluor 610 Alexa Fluor 633 Alexa Fluor 647 Alexa Fluor 660 Alexa Fluor 680 Alexa Fluor 700 Alexa Fluor 750 Alexa Fluor 790 Alexa Fluor 594 610 632 652 663 680 696 752 782 591489 629 648 668 691 702 719 776 804 618506 1285 1200 1300 1100 1150 1400 1300 1750 820QY 0.66 QY 0.33 Cy系列荧光染料 Cy3 Cy3B Cy3.5 Cy5 Cy5.5 Cy7 Cy2Hoechst 33342 (512);550 581 558(625);650 743 675 572;(620) 594;(640) 670 694 767 570;(615) 767 658 1102 792 1128 818 714616 QY 0.15 QY 0.67 QY 0.15 QY 0.28 QY 0.23 QY 0.28 QY 0.12核酸相关探针染料 DAPI 345 Hoechst 33258 345 SYTOX Blue 431 Chromomycin A3 445 Mithramycin 445 YOYO-1 491 Ethidium Bromide 493 Acridine Orange 503 SYTOX Green 504 343TOTO-1, TO-PRO-1Thiazole Orange 455 478 480 575 575 509 620 483530/640523533530617 ~400 1271 394 ~600 624 AT-selective AT-selective DNA CG-selective DNA/RNA DNA AT-selectiveVital stain, TOTO: Cyanine Dimer TO-PRO: Cyanine Monomer 509510536 Propidium Iodide (PI) 668.4472 LDS 7517-AAD543;590546712;607 DNA (543ex/712em), RNA (590ex/607em)7-aminoactinomycin D, CG-selectiveDNA SYTOX Orange 547 TOTO-3, TO-PRO-3 642 DRAQ5 647 细胞功能探针 570 661 647681,697 ~500 413usable excitation down to 488 Fluo-3 DCFH Indo-1DHR361/330 490/405 505 506505 535 526534 855 529 1010346AM ester. Low/High Ca++,AM ester. pH > 6 2'7'Dichorodihydrofluorescein, oxidized formDihydrorhodamine 123, oxidized form, light catalyzes oxidationpH 6/9 SNARF548/579587/635 BR荧光蛋白 Y66F EBFP EBFP2 Y66H 360 380 383 Azurite 383 GFPuv 385 T-Sapphire 399 Cerulean 433 mCFP 433 ECFP 434 CyPet 435 Y66W 436 mKeima-Red 440 TagCFP 458 AmCyan1 458 mTFP1 462 S65A 471 Midoriishi Cyan 472 Wild Type GFP 396,475 S65C 479 TurboGFP 482 TagGFP 482 S65L 484 360Emerald 508 440 448 447 508 511 475 475 477 477 485 620 480 489 492 504 495 508 507 502 505 510 442509511 26k QY0.18 0.27 26 k 0.62 0.40 0.15 0.51 0.24 0.75 0.9 0.77 0.53 0.600.68 15 26 27 13 3 18 3 29 29 54 25 37 34 2039 25 36 64 59 PS0.69 monomer monomer monomer weak dimer monomer weak dimer monomer dimer tetramer dimer dimer dimer monomer weak dimer 487488 weak dimer; (Invitrogen) EGFP S65TAzami GreenZsGreen1TagYFPEYFP 488 492 493 508 514 507 505 505 524 527 26k 105k 26k 0.74 0.91 0.61 0.60 34 41 40 47 51 174 weak dimer 60 tetramer monomer monomerweak dimer Venus Topaz 515 mCitrine 516 YPet 517 TurboYFP 525 ZsYellow1 529 Kusabira Orange 548 mOrange 548 mKO 548 TurboRFP 553 tdTomato 554 TagRFP 555 DsRed monomer 556 DsRed2 (\"RFP\") 563 mStrawberry 574 TurboFP602 574 AsRed2 576 mRFP1 584 J-Red 584 mCherry 587 HcRed1 588 Katusha 588 mKate (TagFP635) 588 TurboFP635 588 mPlum 590 514mRaspberry 528 529 530 538 539 559 562 559 574 581 584 586 582 596 602 592 607 610 610 618 635 635 635 649 527625 0.76 0.77 26 k 0.53 0.65 0.60 0.69 0.60 26 k 0.67 0.69 ~28k 0.1 ~110k 0.55 0.29 26 k 0.35 ~110k 0.21 ~30k 0.25 0.20 0.22 ~52k 0.03 26 k 0.34 0.10 0.57 53 59 80 571.6513 31 49 31 62 95 50 3.5 24 26 26 13 8.8 16 0.6 23 15 22 4.1 13 15 49 49 9 122 98 16 15 13 96 53 monomer weak dimermonomerweak dimerdimer monomer monomer monomer dimer tetramertandem dimer monomer monomer monomerdimer monomer dimer monomer dimer dimer monomer dimer tetramermonomer; faster photobleach than mPlum 598 0.15 其他探针 MonochlorobimaneCalcein 496 380 517 461 623 226Glutathione probepH > 5
Ex: Peak excitation wavelength (nm) Em: Peak emission wavelength (nm) QY: Quantum yield
BR: Brightness; Extinction coefficient * Quantum yield / 1000 PS: Photostability; time to 50% brightness (sec)
