Dynamic and combinatorial synthesis of polymerase

专利名称：Dynamic and combinatorial synthesis of

polymerase primers

发明人：Steven Albert Benner申请号：US11702327申请日：20070205公开号：US08153361B1公开日：20120410

专利附图：

摘要：This invention relates to the field of nucleic acid chemistry, more specifically tocompositions of matter that are nucleic acid analogs, and processes that use them. Stillmore specifically, these compositions comprise two fragments of DNA-like molecules,

each having one or more ends modified to carry a reactive group, where the reactivegroup on one fragment can form a transient covalent bond with the reactive group onthe other under conditions of dynamic equilibrium to form a composite, where thecomposite can then bind to a target oligonucleotide, such as a DNA or RNA molecule.Most specifically, once the transient covalent bond forms, the composite serves as aprimer for a template-directed polymerization using a DNA polymerase, an RNApolymerase, or a reverse transcriptase. Once incorporated, the epimerization causes thebase pair to be destabilized, the duplex containing the epimerized nucleoside to likewisebe destabilized, and the double strand to then disassociate. This leaves the templateavailable to template the synthesis of another complementary oligonucleotidecontaining the epimerizing base.

申请人：Steven Albert Benner

地址：Gainesville FL US

国籍：US

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