Synthetic genomes

专利名称：Synthetic genomes

发明人：J. Craig Venter,Hamilton O. Smith,Clyde A.

Hutchison

申请号：US11635355申请日：20061206

公开号：US200702688A1公开日：20071115

专利附图：

摘要：Methods are provided for constructing a synthetic genome, comprisinggenerating and assembling nucleic acid cassettes comprising portions of the genome,wherein at least one of the nucleic acid cassettes is constructed from nucleic acid

components that have been chemically synthesized, or from copies of the chemicallysynthesized nucleic acid components. In one embodiment, the entire synthetic genome isconstructed from nucleic acid components that have been chemically synthesized, orfrom copies of the chemically synthesized nucleic acid components. Rational methodsmay be used to design the synthetic genome (e.g., to establish a minimal genome and/orto optimize the function of genes within a genome, such as by mutating or rearrangingthe order of the genes). Synthetic genomes of the invention may be introduced intovesicles (e.g., bacterial cells from which part or all of the resident genome has beenremoved, or synthetic vesicles) to generate synthetic cells. Synthetic genomes orsynthetic cells may be used for a variety of purposes, including the generation ofsynthetic fuels, such as hydrogen or ethanol.

申请人：J. Craig Venter,Hamilton O. Smith,Clyde A. Hutchison

地址：Rockville MD US,Reisterstown MD US,Rockville MD US

国籍：US,US,US

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