专利名称：METHOD FOR DETECTING A BIOLOGICALLY
ACTIVE SUBSTANCE IN A LIQUID TO BEANALYSED AND DEVICE FOR REALISING THESAME
发明人：EVDOKIMOV, JURY
MIKHAILOVICH,SKURIDIN, SERGEIGENNADIEVICH,CHERNUKHA, BORISALEXANDROVICH,MIKHAILOV, EVGENYLEONIDOVICH,KOMPANETS, OLEGNIKOLAEVICH,ROMANOV, SERGEINIKOLAEVICH,KOLOSOV, VLADIMIRVASILIEVICH
申请号：EP97913572申请日：19971114公开号：EP0881485A4公开日：20010912
摘要：The present invention allows quickly, simply, precisely and with high sensitivityto determine the presence and concentration of any biologically active substancescapable to interact with linear double-stranded DNA molecules in any liquids includingbiological liquids. The essence of the offered method consists that the lyotropic liquid-crystalline cholesteric DNA dispersion is formed in an aqueous-salted solution of apolymer, neutral in respect to DNA, of the linear double-stranded DNA molecules of alow molecular mass immediately before mixing with an analyzed liquid containing the
determined substance, thereat the liquid for the analysis is prepared by mixing with thespecified polymer under conditions, when the optical properties of the lyotropic liquid-crystalline DNA dispersion are not broken, then through the sample obtained as a resultof mixing of the prepared analysed liquid with the liquid-crystalline DNA dispersion thecircular-polarized light is passed, and the optical signal is registered at two wavelengths,one of them is in the region of the DNA absorption, and another one is in the region ofabsorption of the biologically active substance, then the ratio between these two signalsis calculated, and concentration of the biologically active substance is determined on thisratio using the calibration curve. The offered device contains a wavelength selector (2)having an electrodynamic driver (22) of a positional type and at least one optical element(26, 27, 28) fixed on a motor (33) shaft (35) of the specified driver (22).
申请人：INSTITUT MOLEKULYARNOI BIOLOGII IM. V.A.ENGELGARDTA ROSSIISKOIAKADEMII NAUK,INSTITUT SPEKTROSKOPII ROSSIISKOI AKADEMII NAUK
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