专利名称：Method for replicating or amplifying circular
DNA
发明人：Su'etsugu, Masayuki,Nara, Seia申请号：AU2018228154申请日：20180228
公开号：AU2018228154A1公开日：20190926
摘要：Provided is a method that enables replication or amplification of circular DNA,particularly long-chain circular DNA, in a cell-free system. Specifically, provided is amethod for inhibiting generation of a DNA multimer which is a by-product, duringreplication or amplification of circular DNA having a replication initiation sequence (originof chromosome (oriC)) by using the following enzymes: (1) first enzymes which catalyzereplication of the circular DNA; (2) second enzymes which catalyze an Okazaki fragmentlinking reaction, to synthesize two sister circular DNA molecules forming a catenane; and(3) third enzymes which catalyze a separation reaction of the two sister circular DNAmolecules. Further provided is a method that comprises introduction of oriC into circularDNA by using a transposon.
申请人：OriCiro Genomics, Inc.
代理人：Phillips Ormonde Fitzpatrick
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