激发波长 发射波长 备注 (nm) (nm) 1,5 IAEDANS 336 490 1,8-ANS 372 480 4-Methylumbelliferone 385 502 5-carboxy-2,7-dichlorofluorescein 504 529 5-Carboxyfluorescein (5-FAM) 492 518 5-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH 5-Carboxytetramethylrhodamine (5-TAMRA) 2 568 5-FAM (5-Carboxyfluorescein) 492 518 5-HAT (Hydroxy Tryptamine) 370-415 520-0 5-Hydroxy Tryptamine (HAT) 370-415 520-0 578 604 5-ROX (carboxy-X-rhodamine) 591 567 8 552 5-TAMRA (5-Carboxytetramethylrhodamine) 568 2 6-Carboxyrhodamine 6G 518 3 6-CR 6G 518 3 6-JOE 520 8 7-Amino-4-methylcoumarin 351 430 7-Aminoactinomycin D (7-AAD) 6 7 7-Hydroxy-4-methylcoumarin 360 449, 455 9-Amino-6-chloro-2-methoxyacridine 412, 430 471, 474 ABQ 344 445 Acid Fuchsin 0 630 ACMA (9-Amino-6-chloro-2-412, 430 471, 474 methoxyacridine) Acridine Orange + DNA 502 526 Acridine Orange + RNA 460 650 Acridine Orange, both DNA & RNA 440-480 520-650 Acridine Red 455-600 560-680 Acridine Yellow 470 550 Acriflavin 436 520 Acriflavin Feulgen SITSA 355-425 460 Aequorin (Photoprotein) 466 Photoprotein AFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP 346 442 Alexa Fluor 350™ 441 342 Alexa Fluor 430™ 431 0 Alexa Fluor 488™ 495, 492 519, 520 Alexa Fluor 532™ 531, 532 553, 5 荧光素名称 Alexa Fluor 6™ Alexa Fluor 568™ Alexa Fluor 594™ Alexa Fluor 633™ Alexa Fluor 7™ Alexa Fluor 660™ Alexa Fluor 680™ Alizarin Complexon Alizarin Red Allophycocyanin (APC) AMC, AMCA-S AMCA (Aminomethylcoumarin) AMCA-X Aminoactinomycin D Aminocoumarin Aminomethylcoumarin (AMCA) Anilin Blue Anthrocyl stearate APC (Allophycocyanin) APC-Cy7 APTRA-BTC = Ratio Dye, Zn2+ APTS Astrazon Brilliant Red 4G Astrazon Orange R Astrazon Red 6B Astrazon Yellow 7 GLL Atabrine ATTO-TAG™ CBQCA ATTO-TAG™ FQ Auramine Aurophosphine G Aurophosphine BAO 9 (Bisaminophenyloxadiazole) BCECF (high pH) BCECF (low pH) Berberine Sulphate Beta Lactamase BFP blue shifted GFP (Y66H) 556, 557 577, 578 590, 594 632 7 668 679 530-560, 580 530-560 630, 5 345 345 347 353 555 346 350 345 347 360-381 630, 5 625-650 466/380 424 500 470 520 450 436 465 486 460 450 450-490 365 492, 503 482 430 409 381, 382, 572, 573 603 617, 618 650 666 698 702 580, 624-5 580 655, 660 445 425 444 442 655 442 445 425 444 600 446 655, 660 755 520/530 505 585 0 595 480 490 560 591 550 580 515 395 520,528 520 550 447, 520 445, 447, Ratio Dye, Zn2+ blue shifted GFP Blue Fluorescent Protein BFP/GFP FRET Bimane Bisbenzamide Bisbenzimide (Hoechst) bis-BTC = Ratio Dye, Zn2+ Blancophor FFG Blancophor SV BOBO™ -1 BOBO™ -3 Bodipy 492/515 Bodipy 493/503 Bodipy 500/510 Bodipy 505/515 Bodipy 530/550 Bodipy 2/563 Bodipy 558/568 Bodipy 5/570 Bodipy 576/5 Bodipy 581/591 Bodipy 630/650-X Bodipy 650/665-X Bodipy 665/676 Bodipy Fl Bodipy FL ATP Bodipy Fl-Ceramide Bodipy R6G SE Bodipy TMR Bodipy TMR-X conjugate Bodipy TMR-X, SE Bodipy TR Bodipy TR ATP Bodipy TR-X SE BO-PRO™ -1 BO-PRO™ -3 Brilliant Sulphoflavin FF BTC - Ratio Dye Ca2+ BTC-5N - atio Dye, Zn2+ Calcein Calcein Blue 383 398 360 360 455/405 390 370 462 570 490 533 509 502 528 3 558 5 579 584 625 7 605 504, 505 505 505 528 2 4 4 5 591 588 462 4 430 4/401 459/417 494 373 448 490 461 461 529/505 470 435 481 602 515 9 515 510 7 563 569 570 590 592 2 665 676 511, 513 514 511 7 574 573 570 617 620 616 481 570 520 533/529 517/532 517 440 (Y66H) Blue Fluorescent Protein Ratio Dye, Zn2+ Ratio Dye Ca2+ Ratio Dye, Zn2+ Calcium Crimson™ Calcium Green Calcium Green-1 Ca2+ Dye Calcium Green-2 Ca2+ Calcium Green-5N Ca2+ Calcium Green-C18 Ca2+ 611, 615 531 531 Ca2+ Dye 536 Ca2+ 532 Ca2+ 530 Ca2+ 575 Calcium Orange 9 576 385, 395, 437, 440, Calcofluor White 405 445 Carboxy-X-rhodamine (5-ROX) 576 601 377 420 Cascade Blue™ 398 423 399 399 550 Cascade Yellow 552 400 Catecholamine 410 470 CCF2 (GeneBlazer) CFDA 494 520 430, 433, 474, 475, Cyan Fluorescent CFP - Cyan Fluorescent Protein 436, (453) 476, (501) Protein CFP/YFP FRET Chlorophyll 480 650 Chromomycin A 436-460 470 Chromomycin A 445 575 CL-NERF (Ratio Dye, pH) 504/514 0 Ratio Dye, pH CMFDA 494 520 Ca2+ Dye, Coelenterazine Ca2+ Dye, bioluminescence (429) 465 bioluminescence, native molecule Ca2+ Dye, Coelenterazine cp (Ca2+ Dye,) (430) 442 bioluminescence Ca2+ Dye, Coelenterazine f (437) 473 bioluminescence Ca2+ Dye, Coelenterazine fcp 452 bioluminescence Ca2+ Dye, Coelenterazine h (437) 4 bioluminescence Ca2+ Dye, Coelenterazine hcp (433) 444 bioluminescence Ca2+ Dye, Coelenterazine ip 441 bioluminescence Coelenterazine n (431) 467 Ca2+ Dye, 588, 5 501, 506 506 506/503 506 509 Coelenterazine O Coumarin Phalloidin C-phycocyanine CPM Methylcoumarin CTC CTC Formazan Cy2™ Cy3.1 8 Cy3.5™ Cy3™ Cy5.1 8 Cy5.5™ Cy5™ Cy7™ Cyan GFP cyclic AMP Fluorosensor (FiCRhR) CyQuant Cell Proliferation Assay Dabcyl Dansyl Dansyl Amine Dansyl Cadaverine Dansyl Chloride Dansyl DHPE Dansyl fluoride DAPI Dapoxyl Dapoxyl 2 Dapoxyl 3 DCFDA DCFH (Dichlorodihydrofluorescein Diacetate) DDAO DHR (Dihydorhodamine 123) Di-4-ANEPPS Di-8-ANEPPS (non-ratio) DiA (4-Di-16-ASP) 460 387 384 400-450 4 5 581 514 552 5 9 675 575 470 469 602 506 568 598 566 570 bioluminescence Methylcoumarin 666 695 666 9 670 710, 743 767, 805 433 (453) 475 (501) 500 517 Cell Proliferation 480 520 Assay 453 340 578 337 517 335 518 372 518 336 517 356 none 359 461 403 580 374 574 373 574 504 529 505 463 505 496 488 498 456 535 607 534 705 605 713 591 Dichlorodihydrofluorescein Diacetate (DCFH) DiD - Lipophilic Tracer DiD (DiIC18(5)) DIDS Dihydorhodamine 123 (DHR) DiI (DiIC18(3)) Dinitrophenol DiO (DiOC18(3)) DiR DiR (DiIC18(7)) DM-NERF (high pH) DNP Dopamine DsRed DTAF DY-630-NHS DY-635-NHS EBFP ECFP EGFP ELF 97 Eosin Erythrosin Erythrosin ITC Ethidium Bromide Ethidium homodimer -1 (EthD-1) Euchrysin EukoLight Europium (III) chloride EYFP Fast Blue FDA Feulgen (Pararosaniline) 505 4 4 341 505 9, 551 349 484, 487 748 750 497/510 349 340 558 494 621 634 383 436 535 665 665 415 535 565 501, 502 780 779 0 490-520 583 520 660 6 447 474 Lipophilic Tracer Lipophilic Tracer Ratio Dye, pH Red fluorescent protein Hemicyane label for proteins and DNA Hemicyane label for proteins and DNA Enhanced Blue Fluorescent Protein Enhanced Cyan Fluorescent Protein Enhanced Green Fluorescent Protein Enhanced Yellow Fluorescent Protein 488, 498 507, 516 345 524 529, 532 529 510, 523 528 430 530 5 5, 555 555 595, 605 617 0 513, 520 527, 532 360 494 570 440 520 625 FIF (Formaldehyd Induced Fluorescence) FITC FITC Antibody Flazo Orange 405 490, 494 493 375-530 480 – Fluo-3 506, 506 Fluo-4 494 Fluorescein (FITC) 490, 494 Fluorescein Diacetate 494 Fluoro-Emerald 495 Fluoro-Gold (Hydroxystilbamidine) 361 Fluor-Ruby 555 FluorX 494 FM 1-43™ 479 FM 4-46 515 Fura Red™ (high pH) 572 Fura Red™/Fluo-3 Fura-2, high calcium 335 Fura-2, low calcium 363 Fura-2/BCECF Genacryl Brilliant Red B 520 Genacryl Brilliant Yellow 10GF 430 Genacryl Pink 3G 470 Genacryl Yellow 5GF 430 GeneBlazer (CCF2) GFP (S65T) 498 GFP red shifted (rsGFP) 498 GFP wild type, non-UV excitation (wtGFP) 475 GFP wild type, UV excitation (wtGFP) 395 GFPuv 385 Gloxalic Acid 405 Granular Blue 355 Haematoporphyrin 530-560 Hoechst 33258 345 Hoechst 33342 347 Hoechst 34580 392 HPTS 355 Hydroxycoumarin 325-360 Hydroxystilbamidine (FluoroGold) 361 Hydroxytryptamine 400 Indo-1, high calcium 330 Indo-1, low calcium 346 433 520, 525 517 612 520, 527 516 520, 525 520 524 536 582 520 598 0 657 505 512 590 485 583 475 516 516 509 509 508 460 425 580 487 483 440 465 386-455 536 530 401 475 Excitation ratio dye Excitation ratio dye Emission ratio dye Emission ratio dye Indodicarbocyanine (DiD) Indotricarbocyanine (DiR) Intrawhite Cf JC-1 JO-JO-1 JO-PRO-1 LaserPro Laurodan LDS 751 (DNA) LDS 751 (RNA) Leucophor PAF Leucophor SF Leucophor WS Lissamine Rhodamine Lissamine Rhodamine B LIVE/DEAD Kit Animal Cells Calcein/Ethidium homodimer LOLO-1 LO-PRO-1 Lucifer Yellow Lyso Tracker Blue Lyso Tracker Blue-White Lyso Tracker Green Lyso Tracker Red Lyso Tracker Yellow LysoSensor Blue LysoSensor Green LysoSensor Yellow/Blue Mag Green Magdala Red (Phloxin B) Mag-Fura Red Mag-Fura-2 Mag-Fura-5 Mag-Indo-1 Magnesium Green Magnesium Orange Malachite Green Marina Blue 4 748 360 514 530 532 795 355 3 590 370 380 395 572, 577 577 494 528 566 568 425, 428 373 466 504, 534 490 551 374 442 384 507 524 483/427 369/329 369/330 369/330 369/332 349/328 349/330 506, 507 550 628 362 665 780 430 529 5 4 812 460 712 607 430 465 465 591, 592 592 517 617 580 581 528, 536, 0 422 536 511, 551 516 576 424 505 0 531 600 659/631 508 511/491 505/500 505/482 480/390 480/417 531 575 459 for more details refer to www.probes.com Ratio Dye, Mg2+ Ratio Dye Ca2+ Ratio Dye Mg2+ Ratio Dye, Ca2+ Ratio Dye, Mg2+ Ratio Dye, Ca2+ Ratio Dye, Mg2+ Maxilon Brilliant Flavin 10 GFF Maxilon Brilliant Flavin 8 GFF Merocyanin Methoxycoumarin Mitotracker Green FM Mitotracker Orange Mitotracker Red Mitramycin Monobromobimane Monobromobimane (mBBr-GSH) Monochlorobimane MPS (Methyl Green Pyronine Stilbene) NBD NBD Amine Nile Red Nitrobenzoxadidole Noradrenaline Nuclear Fast Red Nuclear Yellow Nylosan Brilliant Iavin E8G Oregon Green Oregon Green 488-X Oregon Green™ Oregon Green™ 488 Oregon Green™ 500 Oregon Green™ 514 Pacific Blue Pararosaniline (Feulgen) PBFI PE-Cy5 PE-Cy7 PerCP PerCP-Cy5.5 PE-TexasRed [Red 613] Phloxin B (Magdala Red) Phorwite AR Phorwite BKL Phorwite Rev Phorwite RPA Phosphine 3R PhotoResist 450 460 555 360 490 551 578 450 398 398 380 3 466 450 515-555, 559 465 340 2-530 365 460 503 494 503 490, 493 497 506 405 570 340/380 488 488 488 488 488 524 360 370 380 375 465 365 495 495 578 410 516 576 599 470 490 500 461 395 539 530 590, 0 510-650 490-520 580 495 510 522 517 522 514, 520 517 526 455 625 420 670 755, 767 675 710 613 600 430 430 430 430 565 610 Excitation ratio dye Phycoerythrin B [PE] Phycoerythrin R [PE] PKH26 (Sigma) PKH67 PMIA Pontochrome Blue Black POPO-1 POPO-3 PO-PRO-1 PO-PRO-3 Primuline Procion Yellow Propidium Iodid (PI) PyMPO Pyrene Pyronine Pyronine B Pyrozal Brilliant Flavin 7GF QSY 7 Quinacrine Mustard Red 613 [PE-TexasRed] Resorufin RH 414 Rhod-2 Rhodamine Rhodamine 110 Rhodamine 123 Rhodamine 5 GLD Rhodamine 6G Rhodamine B Rhodamine B 200 Rhodamine B extra Rhodamine BB Rhodamine BG Rhodamine Green Rhodamine Phallicidine Rhodamine Phalloidine Rhodamine Red Rhodamine WT Rose Bengal 6-565 565 551 496 341 535-553 433 533 435 539 410 470 (305), 536, 538 412, 415 360 410 0-590 365 560 440 488 571 532 552 550 496, 497 507 470 525 0 523-557 550 0 0 502 558, 2 2 570 530 525, 0 575 578 567 520 376 605 457 574 455 567 550 600 617 561, 5, 570 387 0 560-650 495 510 613 584, 585 716 576 573 520 529 565 555 625 595 605 580 572 527 575, 565 565 590 555 550-600 Chroma R-phycocyanine R-phycoerythrin (PE) rsGFP S65A S65C S65L S65T Sapphire GFP SBFI Serotonin Sevron Brilliant Red 2B Sevron Brilliant Red 4G Sevron Brilliant Red B Sevron Orange Sevron Yellow L sgBFP™ sgBFP™ (super glow BFP) sgGFP™ sgGFP™ (super glow GFP) SITS SITS (Primuline) SITS (Stilbene Isothiosulphonic Acid) SNAFL calcein SNAFL-1 SNAFL-2 SNARF calcein SNARF1 Sodium Green SpectrumAqua SpectrumGreen SpectrumOrange Spectrum Red SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium) 565 473 471 479 484 488 395 340/380 365 520 500 530 440 430 387 387 474 474 336 395-425 365 506/535 508/0 514/3 552/574 578 509 504 507 510 511 511 420 520-0 595 583 590 530 490 450 450 488 509 436 450 460 535/620 3/623 6/630 590/629 576/8 635/587 506, 507 433,/53 497/30, 509/31 559/38, 560 587, 587/35 344 532 480/55 538/44, 524/56 588/48 612, 612/51 443 red shifted GFP (S65T) Excitation ratio dye Quantum’s SuperGlo™GFP AFPs Quantum’s SuperGlo™GFP AFPs Ion Channels Ratio Dye, pH Ratio Dye, pH Ratio Dye, pH Ratio Dye, pH Excitation and emission ratio dye Na+, K+ Vysis Vysis Vysis Stilbene Sulphorhodamine B can C Sulphorhodamine G Extra SYTO 11 SYTO 12 SYTO 13 SYTO 14 SYTO 15 SYTO 16 SYTO 17 SYTO 18 SYTO 20 SYTO 21 SYTO 22 SYTO 23 SYTO 24 SYTO 25 SYTO 40 SYTO 41 SYTO 42 SYTO 43 SYTO 44 SYTO 45 SYTO 59 SYTO 60 SYTO 61 SYTO 62 SYTO 63 SYTO SYTO 80 SYTO 81 SYTO 82 SYTO 83 SYTO 84 SYTO 85 SYTOX Blue SYTOX Green SYTOX Orange Tetracycline Tetramethylrhodamine (TRITC) Texas Red™ Texas Red-X™ conjugate 335 520 470 508, 510 499, 500 488, 491 517, 521 516, 518 488, 494 621 490, 493 512 494 515 499 490 521 420 430 433 436 446 452 622 652 628 652 657 599 531 530 1 3 567 567 445 504 7 390-425 555 595 595 440 595 570 527, 530 522, 519 509, 514 9, 7 6, 555 518, 525 634 507, 527 530 517 535 520 515 556 441 4 460 467 471 484 5 678 5 676 673 619 5 4 560 559 582 583 470 523 570 525-560 576 620 615 Dye for DNA, RNA Dye for DNA, RNA Dye for DNA, RNA Dye for DNA, RNA Dye for DNA, RNA Dye for DNA, RNA Dye for DNA Dye for DNA, RNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Thiadicarbocyanine (DiSC3) 651, 653 674, 675 Thiazine Red R 596 615 Thiazole Orange 510 530 Thioflavin 5 430 550 Thioflavin S 430 550 Thioflavin TCN 350 460 Thiolyte 370-385 477-488 Thiozole Orange 453 480 Tinopol CBS (Calcofluor White) 390 430 TMR 550 573 TO-PRO-1 515 531 TO-PRO-3 4 657 TO-PRO-5 747 770 TOTO-1 514 531, 533 TOTO-3 2 660 TriColor (PE-Cy5) (488) 650 667 TRITC TetramethylRodamineIsoThioCyanate 550 573 True Blue 365 425 TruRed 490 695 Ultralite 656 678 Uranine B 420 520 Uvitex SFC 365 435 wt GFP 395 (475) 508 WW 781 605 639 X-Rhodamine 580 605 XRITC 582 601 Xylene Orange 6 580 Y66F 360 508 Y66H 360 442 Y66W 436 485 Yellow GFP YFP YO-PRO-1 YO-PRO-3 YOYO-1 YOYO-3
wild type GFP Yellow shifted 513 527 Green Fluorescent Protein Yellow Fluorescent 513, 520 527, 532 Protein 491 506 613 629 491 508, 509 612 631
常用荧光染料的激发及发射波长
Fluorescent Dye（荧光染料）Excitation（激发波长nm ）Emission 发射波长nm ） Cy2 TM 4 506 GFP(Red Shifted) 488 507 YO-PRO TM -1 491 509 YOYO TM -1 491 509 Calcein 494 517 FITC 494 518 FluorX TM 494 519 Alexa TM 488 490 520 Rhodamine 110 496 520 ABI,5-FAM 494 522 Oregon Green TM 500 503 522 Oregon Green TM 488 496 524 RlboGreen TM 500 525 Rhodamine Green TM 502 527 Rhodamine123 507 529 Magnesium Green TM 506 531 Calcium Green TM 506 533 TO-PRO TM -1 514 533 TOTO?-1 514 533 ABI,JOE 520 8 BODIPY?530/550 530 550 Dil 9 565 BODIPY?TMR 2 568 BODIPY?558/568 558 568 BODIPY?5/570 5 570 Cy3 TM 550 570 Alexa TM 6 555 570 TRITC 7 572 Magnesium Orange TM 550 575 Phycoerythrin,R B 565 575 Rhodamine Phalloidin 550 575 Calcium Orange TM 9 576
Pyronin Y 555 580 Rhodamine B 555 580 ABI,TAMRA 560 582 Rhodamine Red TM 570 590 Cy3.5 TM 581 596 ABI,ROX 588 608 Calcium Crimson TM 590 615 Alexa TM 594 590 615 Texas Red? 595 615 Nile Red 9 628 YO-PRO TM -3 612 631 YOYO TM -3 612 631 R-Phycocyanin 618 2 C-Phycocyanin 620 8 TO-PRO TM -3 2 660 TOTO?-3 2 660 DiD DilC’(5) 4 665 Cy5 TM 9 670 Thiadicarbocyanine 651 671 Cy5.5 675 694
stbcdaWaveguideGratingSubstrate Surface
