pET28a-ECFP使用说明

编号 北京华越洋VECT90057 载体名称 pET28a-ECFP

pET28a-ECFP pET系列表达载体基本信息： 启动子: 复制子: 终止子: 质粒分类: 原核抗性: 克隆菌株: 培养条件: 表达宿主: 诱导方式: 5'测序引物: 3'测序引物:

T7/lac

ColE1 ori，F1 ori T7 terminator

大肠杆菌载体；PET系列表达质粒 卡那霉素Kan DH5α

37℃，有氧，LB BL21(DE3)

IPTG或乳糖及其类似物 T7:TAATACGACTCACTATAGGG T7-ter:TGCTAGTTATTGCTCAGCGG

pET28a-ECFP载体质粒图谱和多克隆位点信息 pET28a-ECFP载体简介 pET28a-ECFP载体序列

pET28a-ECFP其他相关pET系列表达载体：

pProEX HTC pProEX HTA pMBP-P pMBP-C

pLLP-STII/Plpp-STII pETM-30

pET303/CT-His pET302/NT-His pET-52b pET-51b pET-50b pET-48b pET-47b pET-44c

pET-24 pET-23d pET-23c pET-23b pET-23

pET22b-EBFP pET22b-EGFP pET-22b pET-21d pET-21b pET-21a pET-21 pET-20b pET-19b

pET-44b

pET-44a 非空（BamHI-XhoI） pET-17b pET-14b pET-43.1c pET-43.1b pET-43.1a pET-42c pET-42b pET-42a pET-41c pET-41b pET-41a pET-39b pET-37b pET-35b pET-33b pET-32c pET-32b pET-32a

pET30a-EcoRV pET-30c pET-30b pET28a-SUMO pET28a-EYFP pET28a-DsRed2 pET28a-EGFP pET-29c pET-29b pET-29a pET-28c pET-28b pET-28a pET-27b pET-26b pET-24d pET-24c pET-24b pET-11a

pET-12c pET-12b pET-12a pET-11d pET-11c pET-11b pET-5a pET-5b pET-3d pET-3c pET-3b pET-3a pTrc-CKS pLpp-OmpA pET28a-OFP pET28a-ECFP pET28a-EBFP pProEX HTB pET-Trx pETM-11 pET-His pET-GST pET-DsbA pETBlue-2 pET-40b pET-31b pET-30a

pET28a-mCherry pET-25b pET-24a pET-23a pET-15b pET-21c pET-16b