Procurement, isolation and cryopreservation of mat

专利名称：Procurement, isolation and cryopreservation

of maternal placental cells

发明人：Julie G. Allickson申请号：US11810932申请日：20070605

公开号：US200800098A1公开日：20080313

专利附图：

摘要：Methods, processes and systems for procuring, isolating and cryopreserving atleast one viable, multipotent maternal placental stem cell is provided. Viable maternalplacental stem cells are also provided. The maternal placental stem cell of the invention

expresses the cell surface marker CD117 and at least one of the cell surface markersselected from the group consisting of CD29, CD44, CD73, CD90, CD105, CD166, SSEA-3and SSEA-4 and has low or no expression of at least one of the cell surface markersselected from the group consisting of CD34, CD45, CD133, TRA-1-60 and TRA-1-81. Themethods and process comprise generally obtaining a piece of placental tissue from awhole placenta, disaggregating the placental tissue with mechanical separation orenzymatic digestion, collecting and concentrating placental cells comprising maternalplacental cells with centrifugation and optionally cryopreserving the placental cells.Additional steps for selecting and culturing the maternal placental stem cells is provided.

申请人：Julie G. Allickson

地址：Odessa FL US

国籍：US

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