AZD0865-LCMS-21171-MedChemExpress

Data File D:\\CHEM32\\1\\DATA\\20160725\\BIZ2016-725-WJ13.DSample Name: BIZ2016-725-WJ13=====================================================================Acq. Operator : Guoyuehong Seq. Line : 16Acq. Instrument : HY-LCMS-02 Location : P1-A-05Injection Date : 7/25/2016 16:16:16 Inj : 1 Inj Volume : 3.000 µlAcq. Method : D:\\CHEM32\\1\\METHODS\\1-POS-3MIN.MLast changed : 7/22/2016 09:31:03 by GuoyuehongAnalysis Method : D:\\CHEM32\\1\\METHODS\\METHOD-1\\1-POS-3MIN.MLast changed : 7/25/2016 17:01:18 by Guoyuehong (modified after loading)Method Info : HY-365_5HO1RS,M,A-RP-108，210nm,23min Catalog No : HY-100412 Batch#21171 A-RP-169 Additional Info : Peak(s) manually integrated*DAD1 B, Sig=214,4 Ref=off (20160725\\BIZ2016-725-WJ13.D)200015001000 1.93150000.511.5*DAD1 C, Sig=254,4 Ref=off (20160725\\BIZ2016-725-WJ13.D) 1.594mAU15001250100075025000.51 1.547 1.910 2.082500 2.068 1.594mAU22.5min*1.522.5min* ===================================================================== Area Percent Report ===================================================================== Sorted By : SignalMultiplier : 1.0000Dilution : 1.0000Do not use Multiplier & Dilution Factor with ISTDs Signal 1: DAD1 B, Sig=214,4 Ref=off Signal has been modified after loading from rawdata file! Peak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------| 1 1.594 MM R 0.0390 5432.39014 2321.93726 98.5112 2 1.931 MM R 0.0165 39.66801 40.08915 0.7193 3 2.068 MM R 0.0192 42.43275 4.04875 0.7695 Totals : 5514.49089 2366.07515 HY-LCMS-02 7/25/2016 17:01:29 GuoyuehongPage 1 of 2

Data File D:\\CHEM32\\1\\DATA\\20160725\\BIZ2016-725-WJ13.DSample Name: BIZ2016-725-WJ13 Signal 2: DAD1 C, Sig=254,4 Ref=off Signal has been modified after loading from rawdata file! Peak RetTime Type Width Area Height Area # [min] [min] [mAU*s] [mAU] %----|-------|----|-------|----------|----------|--------| 1 1.547 VV 0.0280 5.20603 2.63722 0.1349 2 1.594 MM R 0.0370 3808.20605 1713.14307 98.7141 3 1.910 MM R 0.0207 25.47864 8.28998e-1 0.6604 4 2.082 MM R 0.0180 18.92314 17.49432 0.4905 Totals : 3857.81386 1734.10360 ===================================================================== *** End of Report ***HY-LCMS-02 7/25/2016 17:01:29 GuoyuehongPage 2 of 2

Data File D:\\CHEM32\\1\\DATA\\20160725\\BIZ2016-725-WJ13.DSample Name: BIZ2016-725-WJ13=====================================================================Acq. Operator : Guoyuehong Seq. Line : 16Acq. Instrument : HY-LCMS-02 Location : P1-A-05Injection Date : 7/25/2016 16:16:16 Inj : 1 Inj Volume : 3.000 µlAcq. Method : D:\\CHEM32\\1\\METHODS\\1-POS-3MIN.MLast changed : 7/22/2016 09:31:03 by GuoyuehongAnalysis Method : D:\\CHEM32\\1\\METHODS\\METHOD-1\\1-POS-3MIN.MLast changed : 7/25/2016 16:59:49 by Guoyuehong (modified after loading)Method Info : HY-365_5HO1RS,M,A-RP-108，210nm,23min Catalog No : HY-100412 Batch#21171 A-RP-169 Additional Info : Peak(s) manually integrated3500003000002500002000001500001000005000000.511.52 11..662413 MSD1 TIC, MS File (D:\\CHEM32\\1\\DATA\\20160725\\BIZ2016-725-WJ13.D) ES-API, Pos, Scan, Frag: 502.5min HY-LCMS-02 7/25/2016 16:59:51 GuoyuehongPage 1 of 2

Data File D:\\CHEM32\\1\\DATA\\20160725\\BIZ2016-725-WJ13.DSample Name: BIZ2016-725-WJ13MS Signal: MSD1 TIC, MS File, ES-API, Pos, Scan, Frag: 50 Spectra averaged over upper half of peaks. Noise Cutoff: 1000 counts. Reportable Ion Abundance: > 10%. Retention Mol. WeightTime (MS) MS Area or Ion 1.621 1114181 368.20 I 367.20 I 1.643 1422657 368.25 I 367.20 I*MSD1 SPC, time=1.586:1.632 of D:\\CHEM32\\1\\DATA\\20160725\\BIZ2016-725-WJ13.D ES-API, Pos, Scan, Frag: 50100806040200100200300400500600700800m/z 367.2Max: 255488*MSD1 SPC, time=1.632:1.684 of D:\\CHEM32\\1\\DATA\\20160725\\BIZ2016-725-WJ13.D ES-API, Pos, Scan, Frag: 50 367.28060200100200300400500600700800m/zHY-LCMS-02 7/25/2016 16:59:51 Guoyuehong 368.340 368.2Page 2 of 2